Part:BBa_K3978041
BL21 vector combined with amoA and hao gene sequence to degrade EE2 in wastewater
Nitrosomonas Europaea ATCC 19718 ammonia oxidizing bacteria convert ammonia to hydroxylamine under the action of AMO and subsequently convert hydroxylamine to nitrite under the action of HAO.
According to existing studies, the EE2 biotransformation obviously depends on the oxidation of NH3, that is, the degradation of EE2 needs to be carried out in the environment of NH3. The possible mechanism is that THE HAO oxidizes NH3 to provide electrons for the oxidation of EE2-OH. Ammonia is present in urine and sewage, so degradation can be achieved without the addition of other substances to the reaction system.
We embed the amoA and hao gene sequence into the petDUET vector, which also contains T7 promoter lac operator to conduct the transcription and translation process. We use the B0030 RBS and B0015 terminator. The AmpR promoter is concluded for selection. The gene sequence have submitted to website numbered in BBa_K3978040 and BBa_K3978040
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1883
Illegal XbaI site found at 30
Illegal PstI site found at 2624 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1883
Illegal PstI site found at 2624
Illegal NotI site found at 911 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1883
Illegal BglII site found at 1067
Illegal BamHI site found at 1318
Illegal XhoI site found at 2786 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1883
Illegal XbaI site found at 30
Illegal PstI site found at 2624 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1883
Illegal XbaI site found at 30
Illegal PstI site found at 2624
Illegal NgoMIV site found at 3103
Illegal NgoMIV site found at 7780
Illegal AgeI site found at 1342 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1400
Illegal BsaI.rc site found at 1781
Illegal BsaI.rc site found at 3688
Illegal SapI.rc site found at 5348
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